Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger

Zheng Lili; Sheng Zhanwu; Ai Binling; Zheng Xiaoyan; Jin Zhiqiang

doi:doi:10.11648/j.sd.20160402.13

| Peer-Reviewed

Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger

Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang

Published in Science Discovery (Volume 4, Issue 2)

Received: 3 May 2016 Published: 4 May 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.

Published in	Science Discovery (Volume 4, Issue 2)
DOI	10.11648/j.sd.20160402.13
Page(s)	70-74
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Mutation, AspergillusNiger, Xylanase,EnzymeActivity

References

[1]	阮同琦,赵祥颖,刘建军.木聚糖酶及其应用研究进展.山东食品发酵，2008(1):42-45。
[2]	Vasimon Ruanglek, Rutchadaporn Sriprang, Nakul Ratanaphan. Cloning, expression, characterization, and high cell-density production of recombinant endo-1,4-β-xylanase from Aspergillus niger in Pichia pastoris [J]. Enzyme and Microbial Technology. 2007, 41(1-2): 19-25.
[3]	徐君飞,张居作.木聚糖酶的应用研究进展[J].农产品加工,2007,（8）:58-59。
[4]	李秀婷.微生物木聚糖酶及在食品工业中的应用[J].农业机械学报,2008,39(2):175-179。
[5]	王修启,邢宝松,张兆敏,等.小麦基础日粮添加不同水平木聚糖酶对猪生产性能的影响[J].华北农学报,2003,18(3):114-116。
[6]	王春雨,邓雪娟,李婷婷,张爱华,杨禄良.饲用木聚糖酶研究进展、开发及产业化[J].饲料与畜牧,2015,(7):46-50。
[7]	赵力超,王燕,刘晓娟,林俊芳.重组多功能木聚糖酶酶解条件的优化[J].农业工程学报，2014，30,(1):270-277。
[8]	郭宁.高效耐酸耐热木聚糖酶及木聚糖酶/甘露聚糖酶双功酶的构建[D].湖南长沙,中南大学，2012,1-69。
[9]	黄运红,龙中儿,李素珍.木聚糖酶高产微生物的筛选和鉴定[J].江西师范大学学报报:自然科学版，2002，6(3):245-248。
[10]	江均平,严自正,张树政.海枣曲霉木聚糖酶降解寡聚木糖的特性[J].生物化学与生物物理学报，1995，27(3):287-92。
[11]	王聪，张光杰.木聚糖酶高产黑曲霉的选育及其酶学性质研究[J].生物技术通报，2010,(6):227-230。
[12]	陈凤莲,朱兴杰,许世玉,方桂珍.黑曲霉的紫外诱变及其液体发酵产木聚糖酶[J].哈尔滨商业大学学报,2009,25(1):47-50。
[13]	李彩霞,房桂干,刘书钗.木聚糖酶酶活测定方法[J].纸和造纸，2011(1):60-61。
[14]	武玉波，冯秀燕.木聚糖酶酶活测定方法的研究[J].中国饲料，2008(4):34-36。

Cite This Article

Plain Text BibTeX RIS

APA Style

Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang. (2016). Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Science Discovery, 4(2), 70-74. https://doi.org/10.11648/j.sd.20160402.13

Copy | Download

ACS Style

Zheng Lili; Sheng Zhanwu; Ai Binling; Zheng Xiaoyan; Jin Zhiqiang. Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Sci. Discov. 2016, 4(2), 70-74. doi: 10.11648/j.sd.20160402.13

Copy | Download

AMA Style

Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang. Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Sci Discov. 2016;4(2):70-74. doi: 10.11648/j.sd.20160402.13

Copy | Download

@article{10.11648/j.sd.20160402.13,
  author = {Zheng Lili and Sheng Zhanwu and Ai Binling and Zheng Xiaoyan and Jin Zhiqiang},
  title = {Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger},
  journal = {Science Discovery},
  volume = {4},
  number = {2},
  pages = {70-74},
  doi = {10.11648/j.sd.20160402.13},
  url = {https://doi.org/10.11648/j.sd.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160402.13},
  abstract = {Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger
AU  - Zheng Lili
AU  - Sheng Zhanwu
AU  - Ai Binling
AU  - Zheng Xiaoyan
AU  - Jin Zhiqiang
Y1  - 2016/05/04
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160402.13
DO  - 10.11648/j.sd.20160402.13
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 70
EP  - 74
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160402.13
AB  - Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.
VL  - 4
IS  - 2
ER  -

Copy | Download

Author Information

Zheng Lili

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China
Sheng Zhanwu

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China
Ai Binling

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China
Zheng Xiaoyan

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China
Jin Zhiqiang

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Science, Haikou, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang. (2016). Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Science Discovery, 4(2), 70-74. https://doi.org/10.11648/j.sd.20160402.13

Copy | Download

ACS Style

Zheng Lili; Sheng Zhanwu; Ai Binling; Zheng Xiaoyan; Jin Zhiqiang. Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Sci. Discov. 2016, 4(2), 70-74. doi: 10.11648/j.sd.20160402.13

Copy | Download

AMA Style

Zheng Lili, Sheng Zhanwu, Ai Binling, Zheng Xiaoyan, Jin Zhiqiang. Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger. Sci Discov. 2016;4(2):70-74. doi: 10.11648/j.sd.20160402.13

Copy | Download

@article{10.11648/j.sd.20160402.13,
  author = {Zheng Lili and Sheng Zhanwu and Ai Binling and Zheng Xiaoyan and Jin Zhiqiang},
  title = {Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger},
  journal = {Science Discovery},
  volume = {4},
  number = {2},
  pages = {70-74},
  doi = {10.11648/j.sd.20160402.13},
  url = {https://doi.org/10.11648/j.sd.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160402.13},
  abstract = {Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Effect of Different Mutation Methods on Xylanase Production Ability by Aspergillus Niger
AU  - Zheng Lili
AU  - Sheng Zhanwu
AU  - Ai Binling
AU  - Zheng Xiaoyan
AU  - Jin Zhiqiang
Y1  - 2016/05/04
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160402.13
DO  - 10.11648/j.sd.20160402.13
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 70
EP  - 74
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160402.13
AB  - Based on the Aspergillus niger AS3.350, the mutants were brewed through inducing five treatments.The effect of different mutation methods on enzyme production ability of Xylanase was disscused and obtainedmutant strain of Xylanase production high ability. The results indicate that: the mutagenesis of UV irradiation and fast neutron irradiation was sensitive to increasing Xylanase activity. UV irradiation seted at 256 nm wavelength,the vertical distance was 20cm,exposure time was 90s, obtained mutant UV256-90 of the emzyme activity was 170.79U/ml,it was 2.18 times of the original strain.The rate 0.0025Gy / s of fast neutron irradiation and vertical distance 12cm,the optimal dose is 0.75Gy, screened out the mutant Fn5-2, the activity is 134.77U/ml,its 1.72 times of the original strain. Mutagenesis by diethyl sulfate, sodium nitrite, and nitrosoguanidine, enzyme activityof the optimal mutant strains were recently starting strain of 1.51 times, 1.46 times, and 1.38 times.Two physical mutagenic methods include ultraviolet mutagenesis and fast neutron irradiation were more sensitive on producing xylanase based on aspergillus Niger.
VL  - 4
IS  - 2
ER  -

Copy | Download