Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)

Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu

doi:doi:10.11648/j.plant.20160405.12

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Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)

Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu

Published in Plant (Volume 4, Issue 5)

Received: 2 September 2016 Accepted: 18 September 2016 Published: 14 October 2016

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Abstract

It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.

Published in	Plant (Volume 4, Issue 5)
DOI	10.11648/j.plant.20160405.12
Page(s)	37-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Botanicals, Crude Extracts, Botrytis fabae, In vitro, In vivo, Vicia faba

References

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. (2016). Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant, 4(5), 37-44. https://doi.org/10.11648/j.plant.20160405.12

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Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016, 4(5), 37-44. doi: 10.11648/j.plant.20160405.12

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016;4(5):37-44. doi: 10.11648/j.plant.20160405.12

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@article{10.11648/j.plant.20160405.12,
  author = {Addisu Tegegn Tola and Meseret Chimdessa Egigu and Bekele Hundie Egdu},
  title = {Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)},
  journal = {Plant},
  volume = {4},
  number = {5},
  pages = {37-44},
  doi = {10.11648/j.plant.20160405.12},
  url = {https://doi.org/10.11648/j.plant.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160405.12},
  abstract = {It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.},
 year = {2016}
}

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TY  - JOUR
T1  - Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)
AU  - Addisu Tegegn Tola
AU  - Meseret Chimdessa Egigu
AU  - Bekele Hundie Egdu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160405.12
DO  - 10.11648/j.plant.20160405.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160405.12
AB  - It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.
VL  - 4
IS  - 5
ER  -

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Author Information

Addisu Tegegn Tola

Pulse and Oil Crops Research Case Team, Sinana Agricultural Research Center, Bale Robe, Ethiopia
Meseret Chimdessa Egigu

Department of Biology, Haramaya University, Haramaya, Ethiopia
Bekele Hundie Egdu

Kulumsa Agricultural Research Center, Kulumsa, Ethiopia

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Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. (2016). Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant, 4(5), 37-44. https://doi.org/10.11648/j.plant.20160405.12

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Addisu Tegegn Tola; Meseret Chimdessa Egigu; Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016, 4(5), 37-44. doi: 10.11648/j.plant.20160405.12

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AMA Style

Addisu Tegegn Tola, Meseret Chimdessa Egigu, Bekele Hundie Egdu. Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.). Plant. 2016;4(5):37-44. doi: 10.11648/j.plant.20160405.12

Copy | Download

@article{10.11648/j.plant.20160405.12,
  author = {Addisu Tegegn Tola and Meseret Chimdessa Egigu and Bekele Hundie Egdu},
  title = {Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)},
  journal = {Plant},
  volume = {4},
  number = {5},
  pages = {37-44},
  doi = {10.11648/j.plant.20160405.12},
  url = {https://doi.org/10.11648/j.plant.20160405.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160405.12},
  abstract = {It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Bio-efficacy of Crude Leaf Extracts of Eucalyptus globulus Against In vitro and In vivo Growth of Chocolate Spot (Botrytis fabae Sard.) of Faba Bean (Vicia faba L.)
AU  - Addisu Tegegn Tola
AU  - Meseret Chimdessa Egigu
AU  - Bekele Hundie Egdu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160405.12
DO  - 10.11648/j.plant.20160405.12
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 37
EP  - 44
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160405.12
AB  - It is widely known that faba bean (Vicia faba L.) is seriously attacked by the fungal disease, chocolate spot, caused by Botrytis fabae resulting in a yield loss ranging from 50 to 100%. Even though synthetic fungicides are used as one of the effective options for the control of plant diseases, the environmental hazards and economic unfeasibility associated with them necessitate the search for relatively safe natural products. This study was initiated to evaluate the antifungal potential of crude extracts of leaves of Eucalyptus globulus, against in vitro and in vivo growth of Botrytis fabae. In the in vitro experiment, antifungal assay was set up using different concentrations of the crude extracts. In vivo experiment was conducted in the field by planting a faba bean variety, Shallo, (EH011-22-1) and selected plants from each plot were used for extract application and subsequent data collection. Laboratory experiment showed that, compared to the control, extracts obtained from each solvent managed to produce statistically significant (p <0.05) inhibition of mycelial growth. Based on the minimum concentration, inhibition from methanol extract (42.2%) significantly varied from that of ethanol (26.3%). Nevertheless, aqueous extract was nearly statistically the same to that of methanol. On the other hand, at maximum concentration (40%), a maximum inhibition percent of 83.7, which was nearly double of that produced by aqueous extract, was recorded from ethanol extract treated plates. Field experiment has shown that the use of ethanol extract of Eucalyptus globulus produced an efficacy of about 58.4%. Number of flowers aborted, number of tillers per m2, number of pods per plant, number of seeds per pod and hundred seeds weight were affected due to the disease suppression effect of the three extracts. However, there was quantitative variation in yield though no statistically significant difference was observed. The result of this study showed that extracts of the tested plant species have natural fungitoxic potential and showed a promising future for the development of safe natural alternative fungicides used for the control of Botrytis fabae after further pertinent tests and screening of the active principles.
VL  - 4
IS  - 5
ER  -

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