Observation of GFP Expression in Pomegranate (<i>Punica granatum</i> L.) Via Pollen-Mediated Transformation

L. Y. Yang; X. Y. Guo; Y. Q. Di; Y. Sun

doi:doi:10.11648/j.plant.20160404.11

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Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation

L. Y. Yang, X. Y. Guo, Y. Q. Di, Y. Sun

Published in Plant (Volume 4, Issue 4)

Received: 25 August 2016 Accepted: 5 September 2016 Published: 22 September 2016

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Abstract

Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.

Published in	Plant (Volume 4, Issue 4)
DOI	10.11648/j.plant.20160404.11
Page(s)	23-28
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

GFP, Pomegranate, Pollen-Mediated Transformation

References

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APA Style

L. Y. Yang, X. Y. Guo, Y. Q. Di, Y. Sun. (2016). Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant, 4(4), 23-28. https://doi.org/10.11648/j.plant.20160404.11

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ACS Style

L. Y. Yang; X. Y. Guo; Y. Q. Di; Y. Sun. Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant. 2016, 4(4), 23-28. doi: 10.11648/j.plant.20160404.11

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AMA Style

L. Y. Yang, X. Y. Guo, Y. Q. Di, Y. Sun. Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant. 2016;4(4):23-28. doi: 10.11648/j.plant.20160404.11

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@article{10.11648/j.plant.20160404.11,
  author = {L. Y. Yang and X. Y. Guo and Y. Q. Di and Y. Sun},
  title = {Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation},
  journal = {Plant},
  volume = {4},
  number = {4},
  pages = {23-28},
  doi = {10.11648/j.plant.20160404.11},
  url = {https://doi.org/10.11648/j.plant.20160404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160404.11},
  abstract = {Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.},
 year = {2016}
}

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TY  - JOUR
T1  - Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation
AU  - L. Y. Yang
AU  - X. Y. Guo
AU  - Y. Q. Di
AU  - Y. Sun
Y1  - 2016/09/22
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160404.11
DO  - 10.11648/j.plant.20160404.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 23
EP  - 28
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160404.11
AB  - Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.
VL  - 4
IS  - 4
ER  -

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Author Information

L. Y. Yang

Department Life Science, Shanxi Normal University, Linfen, P.R. China
X. Y. Guo

Department Life Science, Shanxi Normal University, Linfen, P.R. China
Y. Q. Di

Department Life Science, Shanxi Normal University, Linfen, P.R. China
Y. Sun

The Biotechnology Research Centre，Shanxi Academy of Agricultural Sciences, Taiyuan, P.R. China

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Plain Text BibTeX RIS

APA Style

L. Y. Yang, X. Y. Guo, Y. Q. Di, Y. Sun. (2016). Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant, 4(4), 23-28. https://doi.org/10.11648/j.plant.20160404.11

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ACS Style

L. Y. Yang; X. Y. Guo; Y. Q. Di; Y. Sun. Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant. 2016, 4(4), 23-28. doi: 10.11648/j.plant.20160404.11

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AMA Style

L. Y. Yang, X. Y. Guo, Y. Q. Di, Y. Sun. Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation. Plant. 2016;4(4):23-28. doi: 10.11648/j.plant.20160404.11

Copy | Download

@article{10.11648/j.plant.20160404.11,
  author = {L. Y. Yang and X. Y. Guo and Y. Q. Di and Y. Sun},
  title = {Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation},
  journal = {Plant},
  volume = {4},
  number = {4},
  pages = {23-28},
  doi = {10.11648/j.plant.20160404.11},
  url = {https://doi.org/10.11648/j.plant.20160404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.plant.20160404.11},
  abstract = {Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Observation of GFP Expression in Pomegranate (Punica granatum L.) Via Pollen-Mediated Transformation
AU  - L. Y. Yang
AU  - X. Y. Guo
AU  - Y. Q. Di
AU  - Y. Sun
Y1  - 2016/09/22
PY  - 2016
N1  - https://doi.org/10.11648/j.plant.20160404.11
DO  - 10.11648/j.plant.20160404.11
T2  - Plant
JF  - Plant
JO  - Plant
SP  - 23
EP  - 28
PB  - Science Publishing Group
SN  - 2331-0677
UR  - https://doi.org/10.11648/j.plant.20160404.11
AB  - Pomegranate (Punica granatum L.) is an important fruit crop in the world. Genetic transformation studies of pomegranate have been hindered due to lacking efficient plant regeneration system. At the present study, we explored pollen-mediated transformation for pomegranate where the plasmid DNA harboring GFP was introduced into pollen grains with the aid of sonication. Various sonic parameters showed different effects on pollen grains where the order of effect is ultrasonic intensity > processing duration > treatment times. The observation of GFP in pollen tubes provided the base for potential application of pollen-mediated transformation in pomegranate, which could avoid the tedious tissue culture procedures and easy to apply. And most of all we have provided a novel and efficient approach for pomegranate genetic transformation.
VL  - 4
IS  - 4
ER  -

Copy | Download