Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta

Qingqin Wu; Honghong Song; Juan Feng; Yang Xia; Dezhong Yao

doi:doi:10.11648/j.ijbse.20170506.11

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Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta

Qingqin Wu, Honghong Song, Juan Feng, Yang Xia, Dezhong Yao

Published in International Journal of Biomedical Science and Engineering (Volume 5, Issue 6)

Received: 5 December 2017 Published: 6 December 2017

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Abstract

CLARITY is one new technology which allows the brain tissue become transparent. It has successfully been combined with immunofluorescence staining to achieve the 3D visualization of some molecules or neuronal cells in some disease brains. The temporal lobe epilepsy (TLE) is one neuronal disease which is characterized by the sprouting of the massy fibers (MSF). Previous study has showed that MSF could be affected by the phosphorylation at site-262 of microtubule association protein Tau (p-tau (Ser262)). However, in TLE little useful information was reported concerning the 3D architecture of p-tau (Ser262) and its relationship with 14-3-3 zeta that regulated the phosphorylation of Tau in AD disease. In this paper, pilocarpine-induced epilepsy model was established and identified by Timm-staining. Double immunofluorescent staining results showed that the development of TLE gave rise to the colocalization of p-tau (Ser262) and 14-3-3 zeta protein in CA1 and CA3 zone in hippocampi. The mm-thick brain sections were passively clarified, and 3D reconstruction imaging of the immunofluorescent staining showed that p-tau (Ser262) was diverse cluster-like shape. These results proved that CLARITY could be used to study TLE, in which the 3D morphologic changes of p-tau (Ser262) and the role of 14-3-3 zeta in the regulation of Tau needed to be further investigated.

Published in	International Journal of Biomedical Science and Engineering (Volume 5, Issue 6)
DOI	10.11648/j.ijbse.20170506.11
Page(s)	63-67
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Temporal Lobe Epilepsy, CLARITY, Immunofluorescence, The Phosphorylation of Tau, 14-3-3 Zeta Protein

References

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APA Style

Qingqin Wu, Honghong Song, Juan Feng, Yang Xia, Dezhong Yao. (2017). Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. International Journal of Biomedical Science and Engineering, 5(6), 63-67. https://doi.org/10.11648/j.ijbse.20170506.11

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ACS Style

Qingqin Wu; Honghong Song; Juan Feng; Yang Xia; Dezhong Yao. Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. Int. J. Biomed. Sci. Eng. 2017, 5(6), 63-67. doi: 10.11648/j.ijbse.20170506.11

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AMA Style

Qingqin Wu, Honghong Song, Juan Feng, Yang Xia, Dezhong Yao. Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. Int J Biomed Sci Eng. 2017;5(6):63-67. doi: 10.11648/j.ijbse.20170506.11

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@article{10.11648/j.ijbse.20170506.11,
  author = {Qingqin Wu and Honghong Song and Juan Feng and Yang Xia and Dezhong Yao},
  title = {Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {6},
  pages = {63-67},
  doi = {10.11648/j.ijbse.20170506.11},
  url = {https://doi.org/10.11648/j.ijbse.20170506.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170506.11},
  abstract = {CLARITY is one new technology which allows the brain tissue become transparent. It has successfully been combined with immunofluorescence staining to achieve the 3D visualization of some molecules or neuronal cells in some disease brains. The temporal lobe epilepsy (TLE) is one neuronal disease which is characterized by the sprouting of the massy fibers (MSF). Previous study has showed that MSF could be affected by the phosphorylation at site-262 of microtubule association protein Tau (p-tau (Ser262)). However, in TLE little useful information was reported concerning the 3D architecture of p-tau (Ser262) and its relationship with 14-3-3 zeta that regulated the phosphorylation of Tau in AD disease. In this paper, pilocarpine-induced epilepsy model was established and identified by Timm-staining. Double immunofluorescent staining results showed that the development of TLE gave rise to the colocalization of p-tau (Ser262) and 14-3-3 zeta protein in CA1 and CA3 zone in hippocampi. The mm-thick brain sections were passively clarified, and 3D reconstruction imaging of the immunofluorescent staining showed that p-tau (Ser262) was diverse cluster-like shape. These results proved that CLARITY could be used to study TLE, in which the 3D morphologic changes of p-tau (Ser262) and the role of 14-3-3 zeta in the regulation of Tau needed to be further investigated.},
 year = {2017}
}

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TY  - JOUR
T1  - Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta
AU  - Qingqin Wu
AU  - Honghong Song
AU  - Juan Feng
AU  - Yang Xia
AU  - Dezhong Yao
Y1  - 2017/12/06
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170506.11
DO  - 10.11648/j.ijbse.20170506.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170506.11
AB  - CLARITY is one new technology which allows the brain tissue become transparent. It has successfully been combined with immunofluorescence staining to achieve the 3D visualization of some molecules or neuronal cells in some disease brains. The temporal lobe epilepsy (TLE) is one neuronal disease which is characterized by the sprouting of the massy fibers (MSF). Previous study has showed that MSF could be affected by the phosphorylation at site-262 of microtubule association protein Tau (p-tau (Ser262)). However, in TLE little useful information was reported concerning the 3D architecture of p-tau (Ser262) and its relationship with 14-3-3 zeta that regulated the phosphorylation of Tau in AD disease. In this paper, pilocarpine-induced epilepsy model was established and identified by Timm-staining. Double immunofluorescent staining results showed that the development of TLE gave rise to the colocalization of p-tau (Ser262) and 14-3-3 zeta protein in CA1 and CA3 zone in hippocampi. The mm-thick brain sections were passively clarified, and 3D reconstruction imaging of the immunofluorescent staining showed that p-tau (Ser262) was diverse cluster-like shape. These results proved that CLARITY could be used to study TLE, in which the 3D morphologic changes of p-tau (Ser262) and the role of 14-3-3 zeta in the regulation of Tau needed to be further investigated.
VL  - 5
IS  - 6
ER  -

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Author Information

Qingqin Wu

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Honghong Song

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Juan Feng

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Yang Xia

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China
Dezhong Yao

College of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China

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APA Style

Qingqin Wu, Honghong Song, Juan Feng, Yang Xia, Dezhong Yao. (2017). Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. International Journal of Biomedical Science and Engineering, 5(6), 63-67. https://doi.org/10.11648/j.ijbse.20170506.11

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ACS Style

Qingqin Wu; Honghong Song; Juan Feng; Yang Xia; Dezhong Yao. Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. Int. J. Biomed. Sci. Eng. 2017, 5(6), 63-67. doi: 10.11648/j.ijbse.20170506.11

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AMA Style

Qingqin Wu, Honghong Song, Juan Feng, Yang Xia, Dezhong Yao. Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta. Int J Biomed Sci Eng. 2017;5(6):63-67. doi: 10.11648/j.ijbse.20170506.11

Copy | Download

@article{10.11648/j.ijbse.20170506.11,
  author = {Qingqin Wu and Honghong Song and Juan Feng and Yang Xia and Dezhong Yao},
  title = {Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {5},
  number = {6},
  pages = {63-67},
  doi = {10.11648/j.ijbse.20170506.11},
  url = {https://doi.org/10.11648/j.ijbse.20170506.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20170506.11},
  abstract = {CLARITY is one new technology which allows the brain tissue become transparent. It has successfully been combined with immunofluorescence staining to achieve the 3D visualization of some molecules or neuronal cells in some disease brains. The temporal lobe epilepsy (TLE) is one neuronal disease which is characterized by the sprouting of the massy fibers (MSF). Previous study has showed that MSF could be affected by the phosphorylation at site-262 of microtubule association protein Tau (p-tau (Ser262)). However, in TLE little useful information was reported concerning the 3D architecture of p-tau (Ser262) and its relationship with 14-3-3 zeta that regulated the phosphorylation of Tau in AD disease. In this paper, pilocarpine-induced epilepsy model was established and identified by Timm-staining. Double immunofluorescent staining results showed that the development of TLE gave rise to the colocalization of p-tau (Ser262) and 14-3-3 zeta protein in CA1 and CA3 zone in hippocampi. The mm-thick brain sections were passively clarified, and 3D reconstruction imaging of the immunofluorescent staining showed that p-tau (Ser262) was diverse cluster-like shape. These results proved that CLARITY could be used to study TLE, in which the 3D morphologic changes of p-tau (Ser262) and the role of 14-3-3 zeta in the regulation of Tau needed to be further investigated.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Bring CLARITY to Temporal Lobe Epilepsy: 3D Visualization of p-Tau(Ser262) and 14-3-3 Zeta
AU  - Qingqin Wu
AU  - Honghong Song
AU  - Juan Feng
AU  - Yang Xia
AU  - Dezhong Yao
Y1  - 2017/12/06
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbse.20170506.11
DO  - 10.11648/j.ijbse.20170506.11
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 63
EP  - 67
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20170506.11
AB  - CLARITY is one new technology which allows the brain tissue become transparent. It has successfully been combined with immunofluorescence staining to achieve the 3D visualization of some molecules or neuronal cells in some disease brains. The temporal lobe epilepsy (TLE) is one neuronal disease which is characterized by the sprouting of the massy fibers (MSF). Previous study has showed that MSF could be affected by the phosphorylation at site-262 of microtubule association protein Tau (p-tau (Ser262)). However, in TLE little useful information was reported concerning the 3D architecture of p-tau (Ser262) and its relationship with 14-3-3 zeta that regulated the phosphorylation of Tau in AD disease. In this paper, pilocarpine-induced epilepsy model was established and identified by Timm-staining. Double immunofluorescent staining results showed that the development of TLE gave rise to the colocalization of p-tau (Ser262) and 14-3-3 zeta protein in CA1 and CA3 zone in hippocampi. The mm-thick brain sections were passively clarified, and 3D reconstruction imaging of the immunofluorescent staining showed that p-tau (Ser262) was diverse cluster-like shape. These results proved that CLARITY could be used to study TLE, in which the 3D morphologic changes of p-tau (Ser262) and the role of 14-3-3 zeta in the regulation of Tau needed to be further investigated.
VL  - 5
IS  - 6
ER  -

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