Assessment of the Consciousness Energy Healing Treated <i>Withania Somnifera</i> (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Barry Dean Wellborn; Deborah Lea Smith; Dezi Ann Koster; Elizabeth Patric; Jagdish Singh; Kathleen Starr Vagt; Krista Joanne Callas; Kalyan Kumar Sethi; Parthasarathi Panda; Snehasis Jana

doi:doi:10.11648/j.ajpc.20170602.12

Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, Deborah Lea Smith, Dezi Ann Koster, Elizabeth Patric, Jagdish Singh, Kathleen Starr Vagt, Krista Joanne Callas, Kalyan Kumar Sethi, Parthasarathi Panda, Snehasis Jana

Published in American Journal of Physical Chemistry (Volume 6, Issue 2)

Received: 13 March 2017 Accepted: 28 March 2017 Published: 13 April 2017

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Abstract

Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect^®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (R_t) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at R_t of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Published in	American Journal of Physical Chemistry (Volume 6, Issue 2)
DOI	10.11648/j.ajpc.20170602.12
Page(s)	20-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Ashwagandha, Consciousness Energy Healing Treatment, Biofield Energy Healers, Biofield Energy Healing Treatment, The Trivedi Effect^®, LC-MS, Withanolides, GC-MS, NMR

References

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[28]	Trivedi MK, Patil S, Tallapragada RM (2013) Effect of biofield treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[29]	Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Characterization of physical and structural properties of aluminum carbide powder: Impact of biofield treatment. J Aeronaut Aerospace Eng 4: 142.
[30]	Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) The potential impact of biofield energy treatment on the atomic and physical properties of antimony tin oxide nanopowder. American Journal of Optics and Photonics 3: 123-128.
[31]	Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Jana S (2015) Biofield treatment: A potential strategy for modification of physical and thermal properties of gluten hydrolysate and ipomoea macroelements. J Nutr Food Sci 5: 414.
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APA Style

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, et al. (2017). Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. American Journal of Physical Chemistry, 6(2), 20-30. https://doi.org/10.11648/j.ajpc.20170602.12

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Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Barry Dean Wellborn, et al. Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. Am. J. Phys. Chem. 2017, 6(2), 20-30. doi: 10.11648/j.ajpc.20170602.12

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AMA Style

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, et al. Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. Am J Phys Chem. 2017;6(2):20-30. doi: 10.11648/j.ajpc.20170602.12

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@article{10.11648/j.ajpc.20170602.12,
  author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Barry Dean Wellborn and Deborah Lea Smith and Dezi Ann Koster and Elizabeth Patric and Jagdish Singh and Kathleen Starr Vagt and Krista Joanne Callas and Kalyan Kumar Sethi and Parthasarathi Panda and Snehasis Jana},
  title = {Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy},
  journal = {American Journal of Physical Chemistry},
  volume = {6},
  number = {2},
  pages = {20-30},
  doi = {10.11648/j.ajpc.20170602.12},
  url = {https://doi.org/10.11648/j.ajpc.20170602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpc.20170602.12},
  abstract = {Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.},
 year = {2017}
}

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TY  - JOUR
T1  - Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Barry Dean Wellborn
AU  - Deborah Lea Smith
AU  - Dezi Ann Koster
AU  - Elizabeth Patric
AU  - Jagdish Singh
AU  - Kathleen Starr Vagt
AU  - Krista Joanne Callas
AU  - Kalyan Kumar Sethi
AU  - Parthasarathi Panda
AU  - Snehasis Jana
Y1  - 2017/04/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ajpc.20170602.12
DO  - 10.11648/j.ajpc.20170602.12
T2  - American Journal of Physical Chemistry
JF  - American Journal of Physical Chemistry
JO  - American Journal of Physical Chemistry
SP  - 20
EP  - 30
PB  - Science Publishing Group
SN  - 2327-2449
UR  - https://doi.org/10.11648/j.ajpc.20170602.12
AB  - Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
VL  - 6
IS  - 2
ER  -

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Author Information

Mahendra Kumar Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton

Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi

Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak

Trivedi Global, Inc., Henderson, Nevada, USA
Barry Dean Wellborn

Trivedi Global, Inc., Henderson, Nevada, USA
Deborah Lea Smith

Trivedi Global, Inc., Henderson, Nevada, USA
Dezi Ann Koster

Trivedi Global, Inc., Henderson, Nevada, USA
Elizabeth Patric

Trivedi Global, Inc., Henderson, Nevada, USA
Jagdish Singh

Trivedi Global, Inc., Henderson, Nevada, USA
Kathleen Starr Vagt

Trivedi Global, Inc., Henderson, Nevada, USA
Krista Joanne Callas

Trivedi Global, Inc., Henderson, Nevada, USA
Kalyan Kumar Sethi

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Parthasarathi Panda

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana

Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India

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APA Style

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, et al. (2017). Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. American Journal of Physical Chemistry, 6(2), 20-30. https://doi.org/10.11648/j.ajpc.20170602.12

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ACS Style

Mahendra Kumar Trivedi; Alice Branton; Dahryn Trivedi; Gopal Nayak; Barry Dean Wellborn, et al. Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. Am. J. Phys. Chem. 2017, 6(2), 20-30. doi: 10.11648/j.ajpc.20170602.12

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AMA Style

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Barry Dean Wellborn, et al. Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy. Am J Phys Chem. 2017;6(2):20-30. doi: 10.11648/j.ajpc.20170602.12

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@article{10.11648/j.ajpc.20170602.12,
  author = {Mahendra Kumar Trivedi and Alice Branton and Dahryn Trivedi and Gopal Nayak and Barry Dean Wellborn and Deborah Lea Smith and Dezi Ann Koster and Elizabeth Patric and Jagdish Singh and Kathleen Starr Vagt and Krista Joanne Callas and Kalyan Kumar Sethi and Parthasarathi Panda and Snehasis Jana},
  title = {Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy},
  journal = {American Journal of Physical Chemistry},
  volume = {6},
  number = {2},
  pages = {20-30},
  doi = {10.11648/j.ajpc.20170602.12},
  url = {https://doi.org/10.11648/j.ajpc.20170602.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpc.20170602.12},
  abstract = {Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.},
 year = {2017}
}

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TY  - JOUR
T1  - Assessment of the Consciousness Energy Healing Treated Withania Somnifera (Ashwagandha) Root Extract Using LC-MS, GC-MS, and NMR Spectroscopy
AU  - Mahendra Kumar Trivedi
AU  - Alice Branton
AU  - Dahryn Trivedi
AU  - Gopal Nayak
AU  - Barry Dean Wellborn
AU  - Deborah Lea Smith
AU  - Dezi Ann Koster
AU  - Elizabeth Patric
AU  - Jagdish Singh
AU  - Kathleen Starr Vagt
AU  - Krista Joanne Callas
AU  - Kalyan Kumar Sethi
AU  - Parthasarathi Panda
AU  - Snehasis Jana
Y1  - 2017/04/13
PY  - 2017
N1  - https://doi.org/10.11648/j.ajpc.20170602.12
DO  - 10.11648/j.ajpc.20170602.12
T2  - American Journal of Physical Chemistry
JF  - American Journal of Physical Chemistry
JO  - American Journal of Physical Chemistry
SP  - 20
EP  - 30
PB  - Science Publishing Group
SN  - 2327-2449
UR  - https://doi.org/10.11648/j.ajpc.20170602.12
AB  - Withania somnifera (Ashwagandha) root extract is an important herbal medicine. The current study was designed to investigate the impact of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The LC-MS analysis revealed that the retention time (Rt) of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% was significantly altered. The peak area% of the treated sample at Rt of 5.06, 5.24, 5.49, 5.68, 6.78, 6.87, 8.12, 8.22, 8.36, 8.55, 9.02, and 10.13 minutes were significantly increased in the range of 0.27% to 146.94% compared to the control sample. In addition, the peak area% of the treated sample at Rt of 5.29, 5.36, 6.40, 6.49, 6.56, 6.67, 7.08, 7.37, 7.76, 7.87, 8.02, 8.43, 8.85, 8.97, and 9.12 minutes were significantly decreased in the range of 1.83% to 23.53% compared with the control sample. A total of 18 withanolides such as sitoindoside IX, viscosa lactone B, 24, 25-dihydrowithanolide D, withanolide A, withanone, withaferin A, withanolide D, ixocarpalactone A, withanolide S, withanolide sulfoxide, etc. were proposed with their structure from the molecular mass at m/z 650, 489, 473, 471, 505, and 992 at retention times of 6.87, 7.10, 7.87, 8.22, 8.54, and 9.11 minutes with the help of GC-MS and NMR data of both the control and Biofield Energy Treated samples. The mass peak intensity values of the Biofield Energy Treated sample at the same retention time in was significantly decreased in the range of 3.99% to 52.28% compared with the control sample. These findings suggest that Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha root extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
VL  - 6
IS  - 2
ER  -

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