Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation.
| Published in | American Journal of BioScience (Volume 6, Issue 6) |
| DOI | 10.11648/j.ajbio.20180606.11 |
| Page(s) | 57-64 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Isaria cicadae, Polysaccharides, GC, FTIR Spectra, NMR Spectra
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APA Style
Changsheng Sun, Shijun Yu, Jiayuan Bao, Meizhen Fan. (2019). Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae. American Journal of BioScience, 6(6), 57-64. https://doi.org/10.11648/j.ajbio.20180606.11
ACS Style
Changsheng Sun; Shijun Yu; Jiayuan Bao; Meizhen Fan. Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae. Am. J. BioScience 2019, 6(6), 57-64. doi: 10.11648/j.ajbio.20180606.11
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Download@article{10.11648/j.ajbio.20180606.11,
author = {Changsheng Sun and Shijun Yu and Jiayuan Bao and Meizhen Fan},
title = {Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae},
journal = {American Journal of BioScience},
volume = {6},
number = {6},
pages = {57-64},
doi = {10.11648/j.ajbio.20180606.11},
url = {https://doi.org/10.11648/j.ajbio.20180606.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20180606.11},
abstract = {Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation.},
year = {2019}
}
TY - JOUR T1 - Isolation and Characterization of Water Soluble Polysaccharides from Isaria cicadae AU - Changsheng Sun AU - Shijun Yu AU - Jiayuan Bao AU - Meizhen Fan Y1 - 2019/02/26 PY - 2019 N1 - https://doi.org/10.11648/j.ajbio.20180606.11 DO - 10.11648/j.ajbio.20180606.11 T2 - American Journal of BioScience JF - American Journal of BioScience JO - American Journal of BioScience SP - 57 EP - 64 PB - Science Publishing Group SN - 2330-0167 UR - https://doi.org/10.11648/j.ajbio.20180606.11 AB - Isaria cicadae Miquel is an important medicinal food fungus. The crude polysaccharides from I. cicadae were shown to significantly extend the lifespan of fruit flies in our previous study. To understand the underlying mechanism, the composition and structural characterization of polysaccharides from I. cicadae were determined. A water-soluble crude polysaccharide, obtained from a cultured Isaria cicadae by boiling-water extraction and ethanol precipitation, was fractionated by DEAE-Sepharose column chromatography. The isolate gave four polysaccharide fractions termed B-I-1, B-I-2, B-II-1 and B-II-2. The average ratio of Glu: Man: Gla in B-I-2 was 1.28:1.25:1, B-II-1 was 16.08:1:1.95, and B-II-2, was 1:2.43:2.61. B-I-1 was composed of Ara, Xyl and Glu in the ratio of 1:2.36:33.14. GC and FTIR spectra analysis combined with NMR spectroscopic analysis gave preliminary structures of the four polysaccharide fractions, which may provide an elucidation on the action mechanisms of - polysaccharide on fruit flies proliferation. VL - 6 IS - 6 ER -
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