Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (<i>Colocasia esculenta</i> var. <i>esculenta</i>)

Virendra Mohan Verma

doi:doi:10.11648/j.ajbio.20170506.13

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Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta)

Virendra Mohan Verma

Published in American Journal of BioScience (Volume 5, Issue 6)

Received: 7 October 2017 Accepted: 22 November 2017 Published: 18 December 2017

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Abstract

This is a first report on direct somatic embryogenesis and organogenesis of taro (Colocasia esculenta var. esculenta) using axillary meristem explants. Best somatic embryogenesis was observed in cultures that were established on Murashige and Skoog (1962) (MS medium) containing 10 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2 µM thidiazuron (TDZ) and then transferred on medium with 5 µM TDZ. MS medium containing 3 μM gibberellic acid (GA) and 4.5% sucrose proved best for inducing germination in somatic embryos which converted into 20.0 ± 3.46 complete plantlets per embryo cluster per explant on ½ strength MS basal salts with 1% sucrose. Best organogenesis was observed in cultures that were established on MS medium containing 2 µM TDZ and then transferred on medium with 5 µM TDZ. Subsequent transfer of these cultures on MS medium with 5 µM indole-3-acetic acid (IAA) and 7.5 µM 6-benzylaminopurine (BAP) resulted in maximum shoot multiplication. MS medium containing 2 µM IAA proved best for inducing rooting in multiplied shoots. Both direct somatic embryogenesis and organogenesis resulted in mass and rapid production of taro plantlets which were acclimatized and field transferred. Vigorous plant growth and healthy corm production was observed in the field. This in vitro propagation method of taro through direct somatic embryogenesis and organogenesis is significantly reliable over prevailing methods available for other cultivars and provides sustainable means of quality taro production in the Pacific and the Caribbean region where non-availability of elite seedlings is a limiting issue.

Published in	American Journal of BioScience (Volume 5, Issue 6)
DOI	10.11648/j.ajbio.20170506.13
Page(s)	114-122
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Axillary Meristem, Taro, In Vitro Propagation, Micronesian Region, Direct Somatic Embryogenesis, Direct Organogenesis

References

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APA Style

Virendra Mohan Verma. (2017). Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). American Journal of BioScience, 5(6), 114-122. https://doi.org/10.11648/j.ajbio.20170506.13

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ACS Style

Virendra Mohan Verma. Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). Am. J. BioScience 2017, 5(6), 114-122. doi: 10.11648/j.ajbio.20170506.13

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AMA Style

Virendra Mohan Verma. Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). Am J BioScience. 2017;5(6):114-122. doi: 10.11648/j.ajbio.20170506.13

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@article{10.11648/j.ajbio.20170506.13,
  author = {Virendra Mohan Verma},
  title = {Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta)},
  journal = {American Journal of BioScience},
  volume = {5},
  number = {6},
  pages = {114-122},
  doi = {10.11648/j.ajbio.20170506.13},
  url = {https://doi.org/10.11648/j.ajbio.20170506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20170506.13},
  abstract = {This is a first report on direct somatic embryogenesis and organogenesis of taro (Colocasia esculenta var. esculenta) using axillary meristem explants. Best somatic embryogenesis was observed in cultures that were established on Murashige and Skoog (1962) (MS medium) containing 10 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2 µM thidiazuron (TDZ) and then transferred on medium with 5 µM TDZ. MS medium containing 3 μM gibberellic acid (GA) and 4.5% sucrose proved best for inducing germination in somatic embryos which converted into 20.0 ± 3.46 complete plantlets per embryo cluster per explant on ½ strength MS basal salts with 1% sucrose. Best organogenesis was observed in cultures that were established on MS medium containing 2 µM TDZ and then transferred on medium with 5 µM TDZ. Subsequent transfer of these cultures on MS medium with 5 µM indole-3-acetic acid (IAA) and 7.5 µM 6-benzylaminopurine (BAP) resulted in maximum shoot multiplication. MS medium containing 2 µM IAA proved best for inducing rooting in multiplied shoots. Both direct somatic embryogenesis and organogenesis resulted in mass and rapid production of taro plantlets which were acclimatized and field transferred. Vigorous plant growth and healthy corm production was observed in the field. This in vitro propagation method of taro through direct somatic embryogenesis and organogenesis is significantly reliable over prevailing methods available for other cultivars and provides sustainable means of quality taro production in the Pacific and the Caribbean region where non-availability of elite seedlings is a limiting issue.},
 year = {2017}
}

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TY - JOUR
T1 - Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta)
AU - Virendra Mohan Verma
Y1 - 2017/12/18
PY - 2017
N1 - https://doi.org/10.11648/j.ajbio.20170506.13
DO - 10.11648/j.ajbio.20170506.13
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 114
EP - 122
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20170506.13
AB - This is a first report on direct somatic embryogenesis and organogenesis of taro (Colocasia esculenta var. esculenta) using axillary meristem explants. Best somatic embryogenesis was observed in cultures that were established on Murashige and Skoog (1962) (MS medium) containing 10 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2 µM thidiazuron (TDZ) and then transferred on medium with 5 µM TDZ. MS medium containing 3 μM gibberellic acid (GA) and 4.5% sucrose proved best for inducing germination in somatic embryos which converted into 20.0 ± 3.46 complete plantlets per embryo cluster per explant on ½ strength MS basal salts with 1% sucrose. Best organogenesis was observed in cultures that were established on MS medium containing 2 µM TDZ and then transferred on medium with 5 µM TDZ. Subsequent transfer of these cultures on MS medium with 5 µM indole-3-acetic acid (IAA) and 7.5 µM 6-benzylaminopurine (BAP) resulted in maximum shoot multiplication. MS medium containing 2 µM IAA proved best for inducing rooting in multiplied shoots. Both direct somatic embryogenesis and organogenesis resulted in mass and rapid production of taro plantlets which were acclimatized and field transferred. Vigorous plant growth and healthy corm production was observed in the field. This in vitro propagation method of taro through direct somatic embryogenesis and organogenesis is significantly reliable over prevailing methods available for other cultivars and provides sustainable means of quality taro production in the Pacific and the Caribbean region where non-availability of elite seedlings is a limiting issue.
VL - 5
IS - 6
ER -

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Virendra Mohan Verma

Micronesia Plant Propagation Research Center, Kosrae Agricultural Experiment Station (USDA Landgrant Program), College of Micronesia-FSM, Kosrae, Micronesia

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APA Style

Virendra Mohan Verma. (2017). Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). American Journal of BioScience, 5(6), 114-122. https://doi.org/10.11648/j.ajbio.20170506.13

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ACS Style

Virendra Mohan Verma. Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). Am. J. BioScience 2017, 5(6), 114-122. doi: 10.11648/j.ajbio.20170506.13

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AMA Style

Virendra Mohan Verma. Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta). Am J BioScience. 2017;5(6):114-122. doi: 10.11648/j.ajbio.20170506.13

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@article{10.11648/j.ajbio.20170506.13,
  author = {Virendra Mohan Verma},
  title = {Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (Colocasia esculenta var. esculenta)},
  journal = {American Journal of BioScience},
  volume = {5},
  number = {6},
  pages = {114-122},
  doi = {10.11648/j.ajbio.20170506.13},
  url = {https://doi.org/10.11648/j.ajbio.20170506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20170506.13},
  abstract = {This is a first report on direct somatic embryogenesis and organogenesis of taro (Colocasia esculenta var. esculenta) using axillary meristem explants. Best somatic embryogenesis was observed in cultures that were established on Murashige and Skoog (1962) (MS medium) containing 10 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2 µM thidiazuron (TDZ) and then transferred on medium with 5 µM TDZ. MS medium containing 3 μM gibberellic acid (GA) and 4.5% sucrose proved best for inducing germination in somatic embryos which converted into 20.0 ± 3.46 complete plantlets per embryo cluster per explant on ½ strength MS basal salts with 1% sucrose. Best organogenesis was observed in cultures that were established on MS medium containing 2 µM TDZ and then transferred on medium with 5 µM TDZ. Subsequent transfer of these cultures on MS medium with 5 µM indole-3-acetic acid (IAA) and 7.5 µM 6-benzylaminopurine (BAP) resulted in maximum shoot multiplication. MS medium containing 2 µM IAA proved best for inducing rooting in multiplied shoots. Both direct somatic embryogenesis and organogenesis resulted in mass and rapid production of taro plantlets which were acclimatized and field transferred. Vigorous plant growth and healthy corm production was observed in the field. This in vitro propagation method of taro through direct somatic embryogenesis and organogenesis is significantly reliable over prevailing methods available for other cultivars and provides sustainable means of quality taro production in the Pacific and the Caribbean region where non-availability of elite seedlings is a limiting issue.},
 year = {2017}
}

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